Do i need to have PCIe motherboard for RX6750 XT

Can I Use an Old PCIe Card on a PCIe Slot? - NI pcie 3 slot AMD A620 ATX motherboard with DDR5, PCIe support, dual slots, Q-Latch, 12+2 DrMOS, Wi-Fi 6, Realtek Ethernet, DisplayPort, HDMI™, pcie slot configuration gen1 gen2 gen3 I recently got an LSI 2308, and have not had a chance to test it yet My motherboard only has one PCIE x16 slot ,

pcie slot for gpu List of Motherboards with 3 PCI-Express x16 Slots: GIGABYTE B650 Gaming X AX V2, MSI X670E Gaming Plus WiFi, ASUS ROG Strix Z790-E Gaming WiFi II,